HQD down-regulated the expressions of PDGFra, PDGFrb, PDGFb, PDGFd, COL1A1, COL1A2, COL5A2 and THBS1, and TGF-β and PDGF signaling pathways in the DMN-induced liver fibrosis in rats. The expressions of PDGFra, PDGFrb, PDGFb, PDGFd, COL1A1, COL1A2, COL5A2, and THBS1 were significantly down-regulated by HQD ( P < 0.001). A pathway-gene network was constructed, including 303 DEGs and 52 pathways, and 514 nodes and 2602 edges, among 142 genes with node degrees greater than 10. TGF-beta, ECM-receptor interaction, and the cell adhesion molecules pathways were significantly recovered by HQD ( P < 0.001). ResultsĬollagen deposition and hydroxyproline (Hyp) content were decreased in the HQD group compared with the model group ( P 1.5) and 52 pathways in the HQD group were identified. P values less than 0.05 indicated statistical significance. The expression of candidate genes was verified by qRT-PCR. Pathway-gene and protein–protein interaction (PPI) networks were constructed with Cytoscape software. The liver tissue samples of control group (n = 3), model group (n = 3) and HQD group (n = 3) were examined by microarrays. We analyzed the profiles of differentially expressed genes (DEGs) in dimethylnitrosamine (DMN)-induced liver fibrosis in rat. This study aims to investigate the pharmacological actions of HQD against liver fibrosis in rats by high-throughput gene expression profiling, network analysis and real-time qRT-PCR. Huangqi decoction (HQD) is used for liver fibrosis and cirrhosis treatment in Chinese medicine.
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